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@#[Abstract] Objective: To investigate the effect of long non-coding LINC00969 on proliferation and invasion of breast cancer cell. Methods: Real-time Quantitative polymerase chain reaction (qPCR) was used to detect differential expression of LINC00969 in five breast cancer cell lines (MCF-7, BT-20, MAD-MB-231, ZR-75-1, and SKBR3) , normal breast cells MCF-10A, and in 42 cases breast cancer tissues and adjacent tissues. TMCF-7 cells were transfected with LINC00969 plasmid and empty vector vector vector. The transfection efficiency was verified by qPCR. CCK-8, plate cloning and edu assay were used to detect cell proliferation. Flow cytometry was used to detect cell cycle. Western blotting was used to detect PCNA, CyclinD1, MMP2 and MMP9. Scratch repair test and Transwell test were used to detect cell migration and invasion. Results：Compared with the adjacent tissues, LINC00969 expression in breast cancer tissues was significantly decreased (P<0.05); compared with breast cancer cells MCF-10A, LINC00969 expression in five breast cancer cells was significantly decreased (P<0.05), and the lowest was in MCF-7 cells; overexpression of LINC00969 significantly inhibited the proliferation, colony formation and DNA synthesis of MCF-7 cells (all P<0.05), making MCF-7 cell cycle clear.The ability of wound healing, migration and invasion of the cells were significantly reduced (P<0.05). Overexpression of LINC00969 significantly inhibited the expression of PCNA, cyclinD1, MMP2 and MMP9 (all P<0.05). Conclusion: LINC00969 is low expressed in breast cancer. Overexpression of LINC00969 can inhibit proliferation and migration of breast cancer cell,the mechanism may be related to the abnormal expression of cell cycle and migration related proteins.
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Objective@#To develop a brief version of Quality of Life of College Students Questionnaire(QOLCS-51) to measure the quality of college students conveniently in time.@*Methods@#Qualitative research and two investigations were applied to shorten and verify the brief questionnaire, and SPSS 22.0 and Lisrel 9.20 were used to analyze the reliability and validity of 2 questionnaires.@*Results@#Quality of Life of College Students Questionnaire-bref was developed by deleting 22 items through qualitative research and 6 items by the first investigation. 952 college students from Jiangsu, Anhui and Shanxi Province were selected to participate in the second investigation, which consisted of five domains and 23 items (QOLCS-23). All 23 items were accepted by analysis of difficulty(0.44-0.68), and all have passed the test of critical ratio(P<0.01), the general related index was 0.33-0.60(P<0.01). 78.3% items distinguished the students with/without dyssomnia. Reliability was tested by test-retest reliability coefficient(0.71-0.86), homogeneity reliability coefficient (Cronbach α=0.845) and exploratory factor analysis (6 factors, i.e. physics domain, three subdomains of psychology domain, behavior domain, social domain and environment domain). Validity was tested by correlations of five domains between QOLCS-51 and QOLCS23(greater than 0.8) and confirmatory factor analysis(χ2/df=12.17, RMSEA=0.05, SRMR=0.07, GFI=0.84, AGFI=0.83, CFI=0.92, IFI=0.92, NFI=0.85, NNFI=0.91).@*Conclusion@#QOLCS51 consists of 23 items of QOLCS23, after deleting 28 items, to assess 5 dimensions of physiology, psychology, behavior, environment and social support with good construction validity and criterion-related validity, and good homogeneity reliability and re-test reliability.
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ABSTRACT Heparin-SOD conjugate (Hep-SOD) was prepared by modifying Cu,Zn-SOD with heparin. An acute radiation-induced mouse injury model was constructed to study the radiation protection effects of Hep-SOD conjugate. Fifty-six mice were randomly divided into seven groups: (I) normal control group; (II) irradiated control group; (III) positive control group (amifostine group, 300 mg/kg); (IV) SOD group (35000 U/kg); (V) high dosage of Hep-SOD group (70000 U/kg); (VI) medium dosage of Hep-SOD group (35000 U/kg); (VII) low dosage of Hep-SOD group (17500 U/kg). Drugs were intraperitoneally injected into each mouse 1 h before radiation except for the normal control group. All the irradiated groups were irradiated with 6 Gy. Organ indices, haematopoietic function indices, peripheral blood cells, liver function test, oxidative stress state and pathological observation were detected to study the effects of Hep-SOD on irradiated mice. Results showed that bone marrow suppression of irradiated mice could be reduced when treated by Hep-SOD before radiation. Oxidative stress detection and pathological observation of the liver and intestine showed that the damage caused by radiation was relieved when mice were treated with Hep-SOD before radiation. This study shows a new direction to prevent organisms from the damage caused by radiation.